Inhibition zone experiment of ethylhexylglycerin

1. bacteriostatic carrier pretreatment:

Take Xinhua No. 1 qualitative filter paper and cut it into a number of discs with a diameter of 10 mm; then wrap it tightly with filter paper, sterilize in a pressure steam sterilizer at 121 °C for 20 min; move to oven, 120 °C, 48 h, spare .

2. Medium preparation:

The nutrient agar and the bengal red medium were separately heated and dissolved, and sterilized in a pressure steam sterilizer at 121 ° C for 20 min; when they were cooled to about 50 ° C, they were poured into a sterile Petri dish, and then cooled and solidified. Deposit, spare.

3. Preparation of antibacterial tablets:

Prepare a mixture of ethylhexyl glycerol and methylisothiazolone according to the total concentration, and the proportion of the composite is different. The prepared sterile filter paper disc is immersed therein for 10 min, and air-dried for later use.

4. bacterial suspension preparation:

The microbial strain of the microorganism to be tested is taken, and the bacterial suspension is prepared according to the concentration of bacteria 107 and fungus 106.

5. Antibacterial paper stickers:

Dilute each microbial suspension to be tested with a sterile cotton swab, evenly spread on the prepared plate medium, take 3 pieces of antibacterial tablets and a piece of sterile filter paper, evenly spread on the plate medium, and culture.

6. Measuring the size of the zone of inhibition:

Prepare a series of composite preservatives with the same total concentration of ethylhexyl glycerol and methyl isothiazolinone but different ratios of the two, and the best ratio of ethylhexyl glycerol and methyl isothiazolinone composite preservatives .